DNA Fingerprinting

DNA Fingerprinting and Gel Electrophoresis

10TH GRADE SCIENCE NDS

by EVELYN ROMERO

DNA Fingerprinting Steps

10TH GRADE SCIENCE NDS

DNA Fingerprinting Steps

SCI-10 NDS

Multiple Choice

What is the purpose of gel electrophoresis?

• helps cut DNA

• count the genes in DNA

• separates DNA based on size

• allows for an exact replicated organism

Multiple Choice

What applications can electrophoresis be used for?

• Parental Testing

• Criminal Investigations

• Sequence Endangered Species DNA

• All of the above

Multiple Choice

 Restriction Enzymes . . .

• cut DNA at precise locations

• help bacteria to pick up DNA

• move DNA through a gel

• copy DNA

Multiple Choice

  Which is the primary purpose of using restriction enzymes in gel elctrophoresis?

• cut DNA at precise It allows the strands of DNA to be cut into various lengths for testing

• It restricts the number of base pairs that can be tested in a sample

• It makes the testing simpler by moving the strands into the gel faster

• It charges the DNA strands

Multiple Choice

Which DNA fragment bands move faster and farther in a gel electrophoresis? 

• there is no way to tell 

• the largest ones

• the smallest ones

• none of these

Multiple Choice

 Why do the fragments of DNA in gel electrophoresis travel away from the negative electrode?

• DNA is negatively charged so attracted to the positive end of the unit

• DNA is positively charged to attracted to the negative end of the unit

• the agarose gel in negatively charged

• the agarose gel is positively charged

Multiple Choice

Which two bear species are most closely related?

•  Bear 1 and bear 2

• Bear 1 and Bear 3

• Bear 2 and Bear 3

• There’s no way to tell

Multiple Choice

Shannon does not know who her father is.  There are three possibilities:  Jack, Daniel and Bill.  Who is the father?

• Daniel

• Jack

• Bill

Multiple Choice

Based on these results whose blood was found in the blood stain at the crime scene?

• Bob

• Sue

• John

• Lisa

Multiple Choice

Which suspect is linked to the crime scene by this DNA analysis

• Suspect A

• Suspect B

• Suspect C

• Suspect D

Multiple Choice

Which man is the father of this baby?

• Father 1

• Father 2

• Father 3

• None of them

Multiple Choice

DNA fingerprinting works because

• Most genes are dominant

• All organisms contain RNA

• The most important genes are different among most people

• No two people, except identical twins, have exactly the same DNA 

Multiple Choice

Twelve DNA fingerprints are shown. Which two samples belong to a pair of identical (monozygotic) twins?

• samples 1 and 2

• samples 1 and 6

• samples 1 and 10

• samples 1 and 12

Multiple Choice

3 babies were switched at birth in a hospital. The hospital management team reacts conducting paternity tests. Which baby belongs to each couple?

• Baby 1 belongs to couple A,

  baby 2 to couple C

  &

  baby 3 to couple B

• Baby 1 belongs to couple B,

  baby 2 to couple C

  &

  baby 3 to couple A

• Baby 1 belongs to couple C,

  baby 2 to couple A

  &

  baby 3 to couple C

• Baby 1 belongs to couple A,

  baby 2 to couple B

  &

  baby 3 to couple C