DNA and Gel Electrophoresis

Gel Electrophoresis Lesson

This activities Learning Targets

• Understand the basics of how Gel electrophoresis works.

• Identify the many uses of Gel Electrophoresis.

• Interpret Results of Gel Electrophoresis

Multiple Choice

Gel Electrophorsis is helpful in separating molecules based on their..

• Size

• Shape

• Charge

• Density

Multiple Choice

Overall..DNA has a slight _____ charge.

• Negative

• Positive

Open Ended

Explain why would the DNA move through the gel from negative to positive as noted in the video?

Your previous answer should include something like...

Because the DNA is slightly negatively charged and I have learned that opposite charges attract (from physical science) that the DNA will move from the negative end towards the positive end.

Multiple Choice

To prepare DNA for an electrophoresis trial includes the ______ of DNA by Restrictive Enzymes.

• Chopping up

• Replication

• Enhancement

• Synthesis

Multiple Choice

Restrictive Enzymes will cut DNA at _________ locations.

• Specific

• Random

• Key

• Equal lengthed

Multiple Choice

Based on the video which pieces will move 'fastest' through the gel towards the positive electrodes?

• Shorter Pieces of DNA

• Larger Pieces of DNA

• Less Dense pieces of DNA

• More dense pieces of DNA

Multiple Choice

The dark 'regions' of DNA are called __________.

• DNA Bands

• DNA Markers

• DNA Stripes

• DNA Loci

Multiple Choice

How many DNA bands does the Baby Guppy share with Female #3?

• 1 Band

• 2 Bands

• 3 Bands

• 4 Bands

• 0 Bands

Multiple Choice

How many DNA bands does the Baby Guppy share with Female #2?

• 1 Band

• 2 Bands

• 3 Bands

• 4 Bands

• 0 Bands

Multiple Choice

How many DNA bands does the Baby Guppy share with Female #1?

• 1 Band

• 2 Bands

• 3 Bands

• 4 Bands

• 0 Bands

Open Ended

How does the DNA Bands help us know which Guppy is most likely the mother?

Your answer should have included...

The more DNA bands they share, the more likely they are related.

Multiple Select

Which of these are a use of gel electrophoresis?

• Classifying Organisms

• Solving Crimes

• Paternity Tests

• Producing Clones

• Curing Disease