Gene Cloning

3.3.2 Molecular Cloning & Transgenic Organisms

Multiple Choice

Which is a use of genetically engineered bacteria?

• To identify the remains of an unknown person

• Develop a DNA fingerprint for blood left at a crime scene

• Make human insulin

• Produce clones

Multiple Choice

Several procedures involved in the creation of a transgenic organism are listed.

1. A specific gene of interest is identified in DNA.

2. The DNA fragment is placed in a plasmid.

3. The DNA fragment of interest is isolated.

4. The plasmid is transferred to a bacterial cell.

5. A plasmid is cut by a restriction enzyme.

Which selection represents the correct order of events in creating a transgenic organism?

• 1, 3, 2, 4, 5

• 1, 3, 5, 2, 4

• 3, 5, 2, 4, 1

• 3, 1, 4, 2, 5