Genetic Technology part 2

Multiple Choice

The process of making changes in the DNA code of a living organism is called 

• Selective Breeding
• Inbreeding
• Genetic Engineering
• Di-hybrid Cross

Multiple Choice

What is an advantage of using transgenic bacteria to produce human proteins?

• The bacterial proteins work better
• The proteins can also be used to make plastics
• The bacteria can produce human proteins in large amounts
• The proteins are more durable when made by bacteria

Multiple Choice

Restriction Enzymes come from . . . 

• viruses
• bacteria
• goats
• spiders

Multiple Choice

When using restriction enzymes, what is used to glue together DNA fragments 

• ligases
• plasmids
• bacteriophages

Multiple Choice

In order to insert a human gene into plasmid, both must 

• code for the same gene product.
• be cut by the same restriction enzyme.
• originate from the same type of cell.
• have identical sequences.

Multiple Choice

Restriction Enzymes . . . 

• cut DNA at precise locations
• help bacteria to pick up DNA
• move DNA through a gel
• copy DNA

Multiple Choice

How many restriction sites appear in the picture?

• 1

• 2

• 3

• 4

Multiple Select

Which RE's from above make sticky cuts?

• Alul

• HaeIII

• BamHI

• HindIII

• EcorRI

Multiple Select

Which RE's from above make blunt cuts?

• Alul

• HaeIII

• BamHI

• HindIII

• EcorRI

Multiple Choice

Which of the following is/are true about reverse transcriptase

• works backwards, from DNA to RNA

• works forward from RNA to DNA

• works backwards from RNA to DNA

• works forward from DNA to RNA

Multiple Choice

the function of reverse transcriptase is enzyme used to generate complementary DNA from an RNA template

• false

• true

Multiple Choice

mRNA is converted to cDNA by ………………

• Ligase enzyme
• Reverse Transcriptase enzyme
• Restriction Endonuclease enzyme
• RNA polymerase enzyme

Multiple Choice

What is a cDNA?

• collection of total genomic DNA

• non-coding RNA molecule (containing about 22 nucleotides) found in plants,

• complementary DNA molecules synthesized from mRNA

• small interfering Ribonucleic Acid, is a class of double-stranded RNA molecules

Multiple Choice

What is recombinant DNA

• DNA from 2 different organisms combined

• DNA from 2 different ribosomes

• DNA that is separated out from a vector

Multiple Choice

Which of the following could be used as a vector for a human gene?

• Bacterial Plasmid

• Bacteria

• A mitochondria

• mRNA

Multiple Choice

A circular piece of DNA, found in bacteria, is called:

• A plasmid

• mRNA

• Ribosome

• Nucleotide

Multiple Choice

When DNA is manipulated and moved from one source to another it is known as

• genetic engineering
• electrophoresis
• gene therapy
• GMO

Multiple Choice

Plasmids, yeast, and viruses are known as ____________.

• restriction enzymes
• vectors
• genetically modified organisms
• bacteria

Multiple Choice

The diagram below shows stages in the production of a substance, such as insulin, by genetic engineering. Which line in the table below correctly identifies S and T?

• S - Gene, T - Plasmid

• S - Gene, T - Bacterium

• S - Chromosome, T - Plasmid

• S - Chromosome, T - Bacterium

Multiple Choice

________ are used to ________ DNA pieces by ________

• Restriction enzymes, connect, fission

• Restriction enzymes, isolate, "snipping"

• Plasmids, connect, fission

• Plasmids, isolate, "snipping"

Multiple Choice

Why did scientists edit the genes of bacteria to produce insulin?

• Because the bacteria were diabetic

• Because bacteria have simple DNA that is easier to manipulate, making it easy to produce insulin in a lab

• Because bacteria's complex DNA was perfect for insulin production

• Because insulin has no use outside of a lab setting

Multiple Choice

What does the repressor protein do?

• Allows RNA polymerase to read the gene
• Prevents RNA polymerase from reading the gene
• Reads the gene
• Translates the gene

Multiple Choice

What is an operon?

• a part of the promoter region of a gene
• a part of RNA polymerase
• a unit made up of linked genes that code for proteins needed to do a specific task
• A protein needed for DNA replication

Multiple Choice

In the lac operon, the genes within the operon will be expressed if:

• lactose is absent in the cell
• galactose is present in the cell
• lactose is present in the cell
• glucose is absent in the cell

Multiple Choice

What type of operon is the lac (lactose) operon?

• inducible
• repressible
• positive control
• promoible

Multiple Choice

Which of the following is an example of post-transcriptional control of gene expression?

• the addition of methyl groups to cytosine bases of DNA
• the binding of transcription factors to a promoter
• the removal of introns and splicing together of exons
• gene amplification contributing to cancer

Multiple Choice

In order for a gene to be expressed, ___ must bind to the gene's ___, a specific sequence of nucleotides at one end of the gene, thus initiating transcription.

• DNA polymerase, promotor

• DNA polymerase, operator

• RNA polymerase, promotor

• RNA polymerase, operator

Multiple Choice

What is the inducer in the lac operon?

• cAMP

• glucose

• lactose

• CRP

Multiple Choice

In eukaryotes, proteins that help the RNA polymerase bind to the regulatory region for transcription are called ____________.

• transcription factors
• topoisomerase
• regulatory genes
• activators

Multiple Choice

Gene regulation is the process that is used to:

• help make sugars for cells in plants.

• make glycosomes in cells.

• turn genes on and off.

• Not sure, need help.