Microarrays

Multiple Choice

What is transcribed from DNA to produce a protein and is a good indication of whether or not a particular gene is expressed?

• mRNA

• cDNA

• tRNA

• trRNA

Multiple Choice

What happens after the DNA from the sample is applied to the DNA microarray?

• the mRNA falls off the spot

• the DNA comes apart

• the tRNA will join the complementary strand

• the cDNA will hybridize with the single stranded DNA molecules on the microarray

Multiple Choice

In the microarray, what does the RED color indicate?

• these genes are not expressed or turned off

• these genes are expressed or turned on in cancer

• these genes are expressed in healthy cells

• there are no genes in this cell

Multiple Choice

What does the GREEN color indicate in DNA microarray?

• these genes are expressed in cancer

• these genes are not expressed in cancer

• these genes are not expressed in healthy cells

• there are no genes in these cells

Multiple Choice

What does the YELLOW color indicate in DNA microarray?

• this gene is expressed in cancer cells only

• this gene is expressed in healthy cells only

• there are no genes in the cell

• this gene is expressed in both cancer and healthy cells

Multiple Choice

From microarray data, what can we conclude?

• genes that cause cancer

• which genes may be affected in cancer

• genes that stop cancer

• which cells have genes

Multiple Choice

What does BLACK indicate in dna microarray?

• gene expression in cancer cells

• gene expression in healthy and cancer cells

• no gene expression

• gene expression in healthy cells

Multiple Choice

Which sequence best describes the process for DNA microarray?

• sample prep; cDNA synthesis; hybridization;scan and analysis

• cDNA synthesis; scan and analysis; sample prep; hybridization

• hybridization; cDNA synthesis; scan and analysis

• sample prep; cDNA synthesis; scan and analysis; hybridization

Multiple Choice

Which of the following best describes a microarray?

• A technique used to diagnose cancer

• A way to tell which stage a cancer is currently in

• A form of cancer treatment

• A way to compare gene expression in healthy cells and cancer cells of the same type

Multiple Choice

When a gene is being expressed, what is being produced?

• mRNA which is used to make a protein

• DNA

• New cells

• A tumor

Multiple Select

Which of the following are applications of a microarray?

• Comparing gene expression of healthy cells and cancer cells of the same type

• Diagnosing someone with cancer

• Using expression profiles to decide on a course of treatment for the patient

• Editing genes involved with cancer to cure someone

Multiple Choice

Gene regulation is the process of doing what?

• producing proteins

• replicating DNA

• producing RNA

• determining which genes will be turned on or off

Multiple Choice

What happens after the DNA from the sample is applied to the DNA microarray?

• the mRNA falls off the spot

• the DNA comes apart

• the tRNA will join the complementary strand

• the cDNA will hybridize with the single stranded DNA molecules on the microarray

Multiple Choice

What is transcribed from DNA to produce a protein and is a good indication of whether or not a particular gene is expressed?

• mRNA

• cDNA

• tRNA

• trRNA

Multiple Choice

What happens after the DNA from the sample is applied to the DNA microarray?

• the mRNA falls off the spot

• the DNA comes apart

• the tRNA will join the complementary strand

• the cDNA will hybridize with the single stranded DNA molecules on the microarray

Multiple Choice

What is the complementary cDNA for the following mRNA: AUG CCG UAA

• TAC GGC ATT

• ATC GGC TAA

• UAG GGC AUU

• UAC CCG UAA

Multiple Choice

What is a microarray?

• A musical instrument used in the study of genes

• A type of microscope used to observe gene expression

• A type of small insect found in laboratories

• A laboratory tool used to detect the expression of thousands of genes at the same time

Multiple Choice

Explain the process of hybridization in microarray technology.

• In hybridization in microarray technology, the binding of DNA or RNA sequences to a solid surface is not necessary

• Hybridization in microarray technology is the process of amplifying DNA or RNA sequences using PCR

• The process of hybridization in microarray technology involves the binding of complementary DNA or RNA sequences to a solid surface, followed by detection and analysis of the bound sequences.

• The process of hybridization in microarray technology involves the use of magnetic fields to separate DNA or RNA sequences

Multiple Choice

How are microarrays used to study gene expression?

• By allowing researchers to simultaneously measure the expression levels of thousands of genes.

• By analyzing the DNA sequence of the genes

• By counting the number of chromosomes in the cell

• By measuring the size of the genes

Multiple Choice

Explain the role of microarrays in personalized medicine.

• Microarrays are used to diagnose infectious diseases

• Microarrays help in predicting weather patterns

• Microarrays are used to analyze soil composition

• Microarrays help in identifying specific genetic variations that can inform personalized treatment plans.

Multiple Choice

What is the significance of microarray analysis in cancer research?

• It helps in identifying gene expression patterns in cancer cells and understanding the molecular mechanisms of cancer.

• It is used to determine the impact of social media on cancer research

• It is used to study the effects of climate change on cancer cells

• It helps in identifying the best treatment for cancer patients

Multiple Choice

Discuss the ethical considerations related to the use of microarrays in genetic testing.

• Privacy, consent, discrimination, and psychological impact

• Cost, availability, accuracy, and speed

• Physical health, family history, blood type, and age

• Geographic location, education level, income, and employment status