HTP Midterm Review

Multiple Choice

All of the following are metallic fixatives, EXCEPT:

• Carnoy's fluid

• Helly's Fluid

• Regaud's fluid

• B-5 fixative

Fill in the Blanks

Nuclear fixative are those which preserves the nuclear structure. They usually contain

as their primary component

Multiple Choice

Whare type of fixatives is reommended for GLYCOGEN fixation?

• Mercurial fixatives

• Aldehyde fixatives

• Chromate fixatives

• Alcoholic fixatives

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Give one fixative used for EM.

Fill in the Blanks

What is added to formaldehyde as a preservative?

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Which fixative is highly explosiv when dry?

Multiple Choice

What is the concentration of the alcohol if used as a fixative?

• 70-100%

• 30-50%

• 60-70%

• 50-70%

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What fixative is excellent for fixing dry and wet smears, blood smears, and bone marrow smears?

Fill in the Blanks

What is the most common and fastest decalcifying agent?
(NO ACRONYM, NO NEED TO PUT THE CONCENTRATION)

Multiple Choice

All are commonly used dehydrating agents, EXCEPT

• Alcohol

• Acetone

• Dioxane

• Chloroform

Fill in the Blanks

What alcohol is considered the best dehydrating agent?

Multiple Choice

It is the preferred method for processin whole eye section

• Dry Celloidin

• Wet Celloidin

• Frozen Section

• Paraffin Section

Fill in the Blanks

It is the most rapid and most commonly used clearing agent in HTP

Fill in the Blanks

Clearing agents recommended for CNS and cytological studies?

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Simplest and most common and best embedding medium for routine processing?

Fill in the Blanks

What paraffic wax impregnation method gives the fastest result?

Multiple Choice

What is the best embedding method for EYES

• Dry celloidin

• Wet Celloiding

• Paraffin Wax

• Frozen Section

Fill in the Blanks

A semi-synthetic wax used for embedding the eyes

Multiple Choice

What is double embedding

• Infiltrate --> paraffin

  Embed --> celloidin

• Infiltrate --> gelatin

  Embed --> plastic

• Infiltrate --> celloidin

  Embed --> paraffin

• Infiltrate --> plastic

  Embed --> gelatin

Multiple Choice

A blocking out mold consists of two L-shaped strips with heavy metal brass or metal arranged on a flat metal plate.

• Compound embeding unit

• Tissue Tek

• Peel away

• Leuckhart's embedding mold

Drag and Drop

Cutting angle​

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15 degrees

10 degrees

5 degrees

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Most commonly used adhesive for paraffin section

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What is the refractive index of a glass slide

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Example of Resinous Media​

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Canada Balsam

Clarite

Permount

Glycerin Jelly

Apathy's Gum Arabic

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What component of the aqeous media is used to increase refractive index?

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A 4% aqeous solution in Kraijian's metod for staining AMYLOID tissues, elastic tissue and myelin.

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Blueing is achieved by immersion in​

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Ammonia water

Lithium carbonate

Scott's tape water substitute

4.5% NSS

Saturated picric acid

Fill in the Blanks

What is the best vital dye recommended for mitochondria?