MASTER OF FERMENTATION

- Answer in your group
- Consists of 15 questions.
- 1 minute and 2 points/question

Group 1: Fermentation Topic

​Fermentation Quizes!

Multiple Choice

What is fermentation? 

• Chemical reaction without microorganisms

• Process catalyzed by microorganisms to produce products

• Only spontaneous degradation of food

• Purely synthetic industrial process

Multiple Choice

Which are those not bioproduct from bacterial fermentation

• Bulk chemicals

• Biofuels

• Combustion

• Natural products

Multiple Choice

Who discovered that yeast is a living organism responsible for fermentation?

• Louis Pasteur

• Robert Koch

• Alexander Fleming

• Anton van Leeuwenhoek

Multiple Choice

What type of fermentation uses liquid nutrient media?

• Submerged fermentation (SmF)

• Solid state fermentation (SSF)

• Anaerobic fermentation

• Surface fermentation

Multiple Choice

Define submerged fermentation (SMF).

• Process where microorganisms grow in dry conditions without using any nutrients

•  Process where microorganisms grow in a liquid nutrient medium

• Process where microorganisms grow on solid materials without liquid medium

• A process where microorganisms grow only in the presence of air on the surface

Multiple Choice

Solid-state fermentation (SSF) involves growth of microbes:

• Only under light exposure

• In gaseous phase only

• In liquid medium with air supply

• On solid substrate with minimal free water

Multiple Choice

Which advantage belongs to SSF over SmF?

• Higher cost

• Requires more water

• Cost-effective and concentrated products

• Needs larger fermenters

Multiple Choice

The substrate that is used in solid state fermentation (SSF)....

• Soluble sugar

• Molasses

• Fruit & vegetable waste

• Fruit & vegetable juices

Multiple Choice

Which statement about anaerobic fermentation is TRUE?

• It takes place without O₂ and is slower

• It uses oxygen as a reactant

• It is faster than aerobic fermentation

• It always requires high aeration

Multiple Choice

Aerobic fermentation requires....?

• Absence of oxygen

• Presence of oxygen and agitation

• Dark environment

• Presence of nitrogen gas only

Multiple Choice

What is phase b?

• Log phase

• Lag phase

• Death phase

• Stationary phase

Multiple Choice

Which microorganism is commonly used in acetone–butanol fermentation?

• Saccharomyces cerevisiae

• Bacillus subtilis

• Clostridium acetobutylicum

• Rhizopus oligosporus

Multiple Choice

The typical product ratio in Acetone : Butanol : Ethanol (ABE) fermentation is........

• 2:4:3

• 6:3:1

• 1:3:5

• 3:6:1

Multiple Choice

What is the main limitation of the ABE fermentation industry? 

• Feedstock selection

• High cost and inefficiency of downstream separation

• Lack of fermenter design

• Absence of anaerobic bacteria

Multiple Choice

Which of the following describes the solventogenic phase best?

• pH drops and acids form

• Acids are reassimilated into solvents and pH rises

• Bacteria growth stops

• Only CO₂ forms

​Good Job!!

Now, time for Individual Quiz:
- Consists of 3 questions
- Answer Individually
- 45 secs each

Multiple Choice

What is the main feedstock used in first-generation butanol production?

• Lignocellulosic biomass

• Corn and molasses

• Microalgae

• Seaweed

Multiple Choice

Which enzyme is used to make chocolate with soft centers?

• Lipase

• Amylase

• Invertase

• Protease

Multiple Choice

Which factor distinguishes microbial from chemical synthesis of metabolites?

• Faster reaction rate

• Lower cost

• Biological selectivity and optical purity

• Use of metal catalysts

​- Name of Author