DNA Replication & PCR (2.7, 7.1)

Which of the following statements about DNA replication is TRUE?

• the leading strand is replicated continuously, while the lagging strand is replicated discontinuously
• the leading strand is replicated discontinuously, while the lagging strand is replicated continuously
• both the leading and lagging strands are replicated continuously
• both the leading and lagging strands are replicated discontinuously

Which scientists proved that DNA replication is semi-conservative?

• Hershey and Chase

• Watson and Crick

• Meselson and Stahl

• Franklin and Wilkins

Which radioactive element was used in Meselson and Stahl's experiments?

• ¹⁴C

• ¹⁵N

• ³²P

• ³⁵S

Starting with ¹⁵N¹⁵N (heavy) DNA, and after one generation in the ¹⁴N medium, Escherichia coli cells will contain

• all ¹⁵N¹⁴N (hybrid) DNA.

• half ¹⁵N¹⁵N DNA (heavy) and half ¹⁴N¹⁴N DNA (light).

• half ¹⁴N¹⁴N (light) DNA and half ¹⁵N¹⁴N (hybrid) DNA.

• all ¹⁴N¹⁴N (light) DNA.

Replication proceeds in a ____ to _____ direction.

• 3' to 5'
• 3' to 3'
• 5' to 3'
• 5' to 5'

DNA replication results in

• 2 completely new DNA molecules
• 2 DNA molecules that each contain a strand of the original DNA molecule
• 1 new DNA molecule and 1 original DNA molecule
• 2 DNA molecules, each with original and new parts of DNA interspersed

With regards to DNA replication along the template strand, what event takes place first?

• DNA polymerase begins adding RNA nucleotides
• DNA polymerase begins adding DNA nucleotides
• DNA ligase joins the Okazaki fragments
• primase adds an RNA primer

The enzyme ________ unwinds and unzips the DNA double helix.

• DNA polymerase

• Helicase

• DNA ligase

• Topoisomerase

If DNA polymerase could add bases in the 3' to 5' direction, there would be no need for

• Okazaki fragments

• DNA ligase

• Primase

• Helicase

A scientist isolates a cell line characterized by unusual cell division. In these cells, many small fragments of DNA are found. A defective ____ enzyme may be responsible.

• DNA ligase

• DNA polymerase I

• DNA polymerase III

• Primase

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NGSS.HS-LS3-2