BTB Day One Review

Micropipettors measure small volumes. How many microliters are in 1 milliliter?

• 1
• 10
• 100
• 1000

A micropipettor at the setting in the picture is set to how many microliters?

• 2.5 uL
• 25 uL
• 250 uL
• Impossible to tell from info provided

Which of the following is NOT used to analyze DNA in gel electrophoresis?

• Charge
• Length
• Sequence
• Source

You need to make 50 mL of 1.5% agarose. How many grams of agarose do you need?

• 0.15g
• 0.75g
• 1.5g
• 7.5g

Biotechnology is the study of:

• Living organisms
• Living organisms using tools such as a microscope
• using living organisms as tools
• anything to do with DNA

What is NOT a function of restriction enzymes?

• cut DNA
• Protect bacteria from viruses
• Run DNA in a gel
• Analyze the sequence of DNA

Which of the following is a potential restriction site?

• AATATT
• CATTAC
• TGGGGT
• AAAAAA

What is the benefit of "sticky ends"?

• Easier to find DNA when it is stuck to side of tube
• Easier to use in genetic engineering
• There is no benefit. Have you ever tried to clean up sticky DNA?
• Easier to load a gel

Loading dye is used to do all of the following EXCEPT:

• make DNA sample heavier
• separate into colors so you know approximate location of DNA
• Stain DNA to make it more visible

Restriction enzyme buffers:

• Allow DNA to run through the gel
• Mimic the internal environment of the cell which produced the enzyme
• Cut the DNA
• Cause the bubbles to show in a gel after turning on the power supply