ELISA

Antibodies not only protect us from infections, they can also be used in laboratories to determine whether or not a patient has or has had a disease.

• TRUE
• FALSE

When a positive + result occurs in an ELISA test, then one could conclude that...

• a + result always means illness

• the antibody is present

• it is a false + and no illness

• an enzyme is present

What is a primary antibody?

• the second antibody used to detect foreign particle
• particle produced by the antigen
• the first antibody used to detect foreign particle
• second cell produced by the macrophage

Why is a positive and negative control necessary in the setup of ELISA?

• positive controls are equal to negative controls 
• ELISA is subject to errors; if controls fail the results are untrustworthy
• ELISA is a well run test that typically does not need controls
• negative and positive controls are needed to exclude all results

Why is it necessary to wash the samples repeatedly?

• it is important to keep the lab wells clean
• it is important to wash away all bound and unbound antibodies from teh wells
• it is important to wash away the unbound antibodies
• washing the wells is just solid lab practice

A serial dilution is 

• the stepwise saturation of a substance in solution.
• a dilution that increase by a factor of 1/10 every step
• the stepwise dilution of a substance in solution.
• a series of sequential dilutions used to increase a dense culture of cells to a more usable concentration.

What would be the tube dilution?

• 1/10
• 2/10
• 1/100
• 1/1000

What would be the final dilution?

• 1/10
• 1/100
• 1/1000
• 1/10000

What does the symbol μL stand for?

• nanoliters
• milliters
• microliters
• muliters

From what cells do antibodies originate?

• b cells
• t cells 
• prison cells
• red blood cells