AMGEN Overview

A protein that can cut DNA at a specific sequence

• DNA ligase
• DNA polymerase
• Restriction Enzymes
• Plasmid

You can use Ligase to join DNA together regardless of the restriction enzyme used to cut them apart

• True
• False

Asymetrical ends of DNA after being cut by a restriction enzyme

• Competent Ends
• Sticky Ends
• Ligated Ends
• Restrictive Ends

A circular molecule of DNA

• Double Helix
• Nuclear DNA
• Supercoiled
• Plasmid

E.Coli transformed with the pARA--R plasmid can grow on ampicillin and LB media because it contains an ampicilin resistant gene

• True
• False

DNA that contains a gene sequence from two or more sources

• Plasmid
• Competent Cell
• Recombinant DNA
• Proteins

A lab tool that uses positive charge to septette DNA by size

• Gel Electrophoresis
• Micropipette
• Restriction Enzymes
• Recombinant DNA

When you run a sample on a gel that contains just plasmid DNA you will always get just one band that corresponds to the BP

• True
• False

A student Conducting the conducting the AMGEN labs receives the results in the picture.  Did he/she successfully transform the bacteria?

• Yes
• No

A student Conducting the conducting the AMGEN labs receives the results in the picture.  Did he/she successfully transform the bacteria?

• Yes
• No