pGLO Lab Quiz

Which protein is responsible for allowing the bacteria to grow in the presence of ampicillin?

• GFP
• araC
• Beta Lactamase
• Restriction Enzyme

What is the name of the plasmid we used for lab?

• GFP
• bla
• araC
• pGLO

Which gene is responsible for making the bacteria resistant to ampicillin?

• bla
• pGLO
• araC
• GFP

 What was used to cut the plasmid to enable us to insert the desired gene?

• ligase
• restriction enzymes
• GFP
• pGLO

What did we use to create temperature differential to sweep plasmid inside

• heat shock
• ice
• arabinose
• CaCl₂

Tags

NGSS.HS-PS1-5

Which plate had bacterial growth and bacterial GLOWth?

• LB
• LB/Amp
• LB/Amp/Ara
• All

What is used to ensure the bacteria transformed with the gene for antiobiotic resistance as only the transformed bacteria will grow on this medium?

• ampicillan
• arabinose
• LB
• GFP

Which gene is responsible for the bacteria glowing?

• bla
• araC
• GFP
• pGLO

Tags

NGSS.HS-LS1-1

Which gene is responsible for "turning on" the glow?

• bla
• ARAc
• GFP
• LB

What abbreviation represents the sugar molecule needed to "turn on" the green fluorescent protein gene?

• LB
• AMP
• ARA
• +pGlo