Special Stain Review

In the Masson Trichrome stain with aniline blue, collagen stains what colour? 

• Blue
• Yellow
• Red
• All of the above

Alcian Blue is used at pH 1 to demonstrate:

• sulfated mucopolysaccharides
• neutral mucins
• glycogen
• acid mucopolysaccharides

The “streaming” artifact associated with glycogen is: 

• is caused by alcohol fixation
• prevented by using formalin fixationcaused by insufficient fixation 
• is a fixation artifact
• caused by insufficient fixation

What is a true leuco dye?

• A true leuco dye is considered part of the physical theory of staining. 
• A true leuco dye relies on the formation of a chromophore. 
• A true leuco dye incorporates silver salts. 
• A true leuco dye relies on the production of an auxochrome

Mucicarmine is an important CHO stain for the demonstration of:

• mucin pigments
• adenocarcinomas of the GI tract
• acid mycopolysaccharides
• glucose

What is the function of iron alum in the reticulin stain?

• reducer
• oxidizer
• sensitizer
• toner

Masson Fontana uses what as a reducer?

• formalin
• ammonia
• potassium permanganate
• melanocytes

In the Gordon & Sweet reticulin stain, the silver is:

• soluble in the ammoniacal silver 
• oxidized to a metallic silver
• reduced to a metallic silver
• referred to as a methenamine silver

Gomori’s Methenamine Silver (GMS) is classified as an induced argentaffin reaction. This means that: 

• Formalin must be used as a reducer
• No extraneous reducer is needed
• fungi are able to attract silver and reduce it once the CHOs in their cell walls are exposed
• It does not need to be toned

The purpose of using nonmetallic forceps, acid-cleaned glassware and distilled water in silver techniques are to prevent:    

•  Non-selective silver staining
•  Changes in pH of silver solution
• False negative staining 
• Inability to tone with gold chloride