Genetic Engineering

Restriction Enzymes . . . 

• cut DNA at precise locations
• help bacteria to pick up DNA
• move DNA through a gel
• gene copy DNA

Restriction Enzymes come from . . . 

• viruses
• bacteria
• goats
• spiders

Why do bacteria have restriction enzymes?

• To prevent being infected by viruses
• To help DNA get into the cell
• To be able to grow in an incubator
• They don't - viruses have restriction enzymes

What shape are plasmids?

• linear
• circular
• double helix
• square

What do restriction enzymes recognize in order to cut the DNA into pieces?

• palindrome sequences of bases
• repeating sequences of bases
• specific genes in the DNA
• their homologous pair

A molecule containing DNA from two different organisms is called

• a plasmid.
• a polyploid.
• a hybrid organism.
• recombinant DNA.

Tags

NGSS.HS-LS3-2

How do EcoRI and BamHI differ?

• different blunt ends
• different restriction sites
• produce different proteins
• cut DNA 

Recombines DNA fragments 

• ligases
• plasmids
• bacteriophages

Originates from bacterial DNA and has the ability to cut DNA.  Often referred to as DNA scissors.  

• Sample Loading Buffer (SLB)
• Electrophoresis Buffer 
• Restriction Enzymes

A length of unpaired bases thatstick out.  These have been cut from a specific bases in a specific sequence of bases.  

• Sticky Ends 
• Recombinant DNA 
• DNA size standard
• blunt ends