Genetic Engineering + pGlo + Enzymes

Which protein is responsible for allowing the bacteria to grow in the presence of ampicillin?

• GFP

• Arabinose

• Beta Lactamase

• Restriction Enzyme

What is the name of the plasmid we used for lab?

• GFP
• bla
• araC
• pGLO

 What was used to cut the plasmid to enable us to insert the desired gene?

• ligase
• restriction enzymes
• GFP
• pGLO

What did we use to make sure the pGlo plasmid got inside the bacteria

• heat shock

• ice

• arabinose

• GFP gene

Which plate had bacterial colonies that grew on ampicillin?

• -pGlo

• -pGlo AMP

• +pGlo AMP

• All

In order for the bacteria to glow, what molecule had to be added to the +pGlo colonies?

• ampicillin

• GFP gene

• Arabinose

• no molecule needed

Which of the plates would show no growth?

• -pGlo, -AMP

• -pGlo, +AMP

• +pGlo, +AMP

• all would show growth

What is the circular piece of DNA found in bacteria called?

• homologous chromosome
• sister chromatid
• plasmid
• arabinose

Which sequence of DNA strands below would be cut by the restriction enzyme EcoRI?

• GAACCC

  CTTGGG

• GAATTC

  CTTAAG

• GTAAGT

  CATTCA

• CATTCA

  GTAAGT

Which of the plates would show a LAWN of growth?

• -pGlo, -AMP

• -pGlo, +AMP

• +pGlo, +AMP

• none would show a LAWN