Microbial Staining

Which of the following is not a simple stain

• Methylene blue

• Iodine

• Crystal Violet

• Safranin

Which of the following is a mordant

• Methylene blue

• Iodine

• Crystal Violet

• Safranin

1st step of the gram stain procedure

• Crystal Violet

• Iodine

• Alcohol

• Safranin

2nd step of the gram stain procedure

• Crystal Violet

• Iodine

• Alcohol

• Safranin

3rd step of the gram stain procedure

• Crystal Violet

• Iodine

• Alcohol

• Safranin

4th step of the gram stain procedure

• Crystal Violet

• Iodine

• Alcohol

• Safranin

When finished gram staining, G+ bacteria appear

• Purple

• Pink

• Green

• Yellow

When finished gram staining, G- bacteria appear

• Purple

• Pink

• Green

• Yellow

Acid Fast staining and gram staining are both considered to be

• Differential stains

• Selective stains

• Simple stains

• Negative stains

Acid fast staining is used for organisms that contain ________ in their cell walls

• Peptidoglycan

• Flagella

• Wax

• Carbohydrates