PCR review Questions

name the thermostable enzyme used in pcr.

• taq polymerase

• dna ligase

• dna polymerase

• rna polymerase

which bacterium produces taq polymerase?

• E.coli

• vibrio choleriae

• Thermus aquaticus.

• Agrobacterium

PCR is used to -

• extract dna

• colour dna

• purify dna

• amplify DNA

What is the purpose of PCR?

• To produce millions of copies of DNA.
• To produce millions of copies of a specific region of DNA
• To add nucleotides to a DNA sequence
• To watch polymerase work.

What does PCR stand for?

• Polymerase Chronic Reagent
• Principle Chorionic Reliability
• Polymerase Chain Reaction
• Probably Cannot React

IN PCR why is the DNA heated?

• To split the DNA strand

• To remove the DNA strand

• To correct the DNA strand

From where does Taq polymerase come?

• Bacteria that live in hydrothermal vents/hot springs.
• Cambodia
• The rain forest
• The CDC

What happens in the Denature step of PCR?

• The DNA nucleotides are broken apart.
• The base-pairing rules for DNA are reversed.
• The double-stranded DNA is separated into two single strands of DNA.
• The DNA is returned to its natural setting.

What happens during the Anneal step of PCR?

• Primers are created.
• The primers attach to the target DNA region.
• Primers copy the new DNA strand.
• Primers sequence DNA

What is the purpose of the Extension step of PCR?

• To create the primers.
• To extend the time it takes to produce DNA.
• To allow polymerase to create the complementary strands of DNA.
• To allow substrates to copy DNA sequences.