CRISPR-CAS9 and gene editing

A vector in genetic engineering is

• plasmid or virus that contains or carries modified genetic material

• a host organism that is used to grow up lots of copies of transfected DNA

• an organism that can carry a disease to a host

• CRISPR is an example

The transfer of genes into the non-germline cells of the patient is called

• Somatification

• Somatic cell gene therapy

• basal cell gene therapy

• somatic cell therapy

When a vector with the gene interest is placed directly into the affected tissues it is called

• In vivo

• in situ

• ex vivo

• ex situ

When cells are removed from the body, incubated with the vector and gene-engineered cells are then returned the body, this is genetic engineering

• Ex vivo

• In situ

• In vivo

• Ex situ

The first reported case of gene therapy was in 1999 ________

• and was done to Jesse Gelsinger, an 18-year-old with an X-linked genetic disease involving the metabolism of ammonia.

• by Dr. Jennifer Doudna using CRISPR-Cas9 on herself

• by Chinese scientist Jianku He to embryos to disable their HIV receptors

• by Dr. Victoria Ashton on a patient with cystic fibrosis

CRISPR can change DNA in humans and animals.  What did scientists look at to help them create CRISPR?

• Sheep cloning
• Palindromic repeats
• Common Cold
• Bacteria fighting off viruses

What is one way CRISPR has already been used to change genes?

• Clone sheep
• Give people superpowers
• Cure animal diseases
• Military purposes

Tags

NGSS.HS-LS3-2

Which option most closely relates to how CRISPR-Cas9 works?

• "measure and cut"

• "cut and paste"

• "seek and destroy"

• "trash and burn"

What enzyme is used in CRISPR gene editing?

• Cas3

• Cpr 9

• Rna5

• Cas 9

How does CRISPR differ from previous techniques of gene editing?

• slower, more expensive, but more accurate
• faster, cheaper, and more accurate
• faster, more expensive, more accurate
• slower, cheaper, less accurate