DNA Technology

What is the purpose of PCR?

• To produce millions of copies of DNA.
• To produce millions of copies of a specific region of DNA
• To add nucleotides to a DNA sequence
• To watch polymerase work.

What does PCR stand for?

• Polymerase Chronic Reagent
• Principle Chorionic Reliability
• Polymerase Chain Reaction
• Probably Cannot React

At what temperature does the Denature step of PCR occur?

• 94-96° C
• 50-65° C
• 72° C
• Any temperature

Tags

NGSS.HS-PS1-2

NGSS.HS-PS1-5

At what temperature does the Anneal step of PCR occur?

• 94-96° C
• 50-65° C
• 72° C
• Any temperature

At what temperature does the Extension step of PCR occur?

• 94-96°C
• 50-65°C
• 72°C
• Any temperature

What is the function of a primer?

• To identify the particular region of DNA to be copied by PCR.
• To copy DNA.
• To create DNA nucleotides.
• To maintain the temperature of the PCR reaction.

What are the base-pairing rules in DNA?

• A-C, G-T
• A-U, G-C
• A-T, G-C
• A-C, G-U

Which of the following best describes the role of primers?

• Frame the beginning and end of the target sequence
• Copy the DNA 
• Cause DNA strands to separate
• Heat the sample

PCR happens in 3 steps (denaturing, annealing, extending) or 1 "cycle". How many times are cycles typically repeated?

• 1
• 10
• 30
• Over 1 billion

You decide to use PCR to determine if you have Cystic Fibrosis, a genetic disorder. You then decide to also check if you are at risk for developing Alzheimers, another genetic disorder.
What PCR "ingredient" would be different in these two tests?

• Template DNA
• Nucleotides
• Taq DNA polymerase
• Primers