pGLO

What is pGlo?

• A plasmid that dies in the presence of ampicillin

• A gene for green fluorescent protein (GFP)

• A plasmid with genes for GFP and ampicillin resistance

• A chemical that makes jelleyfish glow

The purpose of the Petri dish with pGlo and ampicillin is to...

• Fight off invading bacteria
• Identify transformed bacteria
• Kill all bacteria so the dish is sterile
• Destry extra plasmids

Why do you not add plasmid to the -pGlo tube?

• The plasmid was already added to the -pGlo tube before
• Instructions are wrong; you should add the plasmid in to the - tube
• The -pGlo tube is a control, with bacteria but no plasmid

On which plate would you find bacteria most like the original non-transformed E. coli?

• -pGlo, LB

• -pGlo, + AMP, LB

• +pGlo, +AMP, LB

• +pGlo, LB

What is the point of the heat shock and transformation solution?

• To kill bacteria we don't want
• To make the bacteria's membrane open so the pGlo can go in
• To heat the plasmid up so ligase will work
• To make the pGlo plasmid

How many of your plates will have the -pGlo bacteria on them?

• 1
• 2
• 3
• 4

Which plate do you predict will have bacterial growth and bacterial GLOWth?

• LB-

• LB/Amp/+

• LB/Amp/-

• LB +

What is used to ensure the bacteria transformed with the gene for antiobiotic resistance as only the transformed bacteria will grow on this medium?

• ampicillan

• calcium chloride

• LB

• GFP

What is the circular piece of DNA found in bacteria called?

• homologous chromosome

• sister chromatid

• plasmid

• restriction enzyme

What did we use to interact with the negative charges of the negatively charged phosphates on DNA helix and negative head of lipid molecules in the cell membrane?

• heat shock

• ice

• CaCl₂

• luria broth