PAM and CRISPR

What did CRISPR originally do?

• defend bacteria against viruses

• create viruses

• create bacteria

• defend viruses from bacteria

What do CAS1 and CAS2 do?

• Identify deadly viruses

• Identify an invader's DNA and remove it

• Create RNA to kill invaders

• Directly kill invaders through endocytosis

What is the protospacer?

• a type of DNA that functions in a phage infection

• the DNA of the original bacteria

• DNA that is mutated through UV rays

• Nothing I need to worry about.

What does CAS9 form with the two different types of RNA?

• A macrophage

• A bacteriophage

• a search complex

• a virus

What can CAS9 do?

• kill an invading bacteriophage

• kill viruses by endocytosis

• kill the bacteria itself

• prevent infection through the first line of defense

What is PAM?

• a 2-6 base pair sequence that signals infection

• a macrophage

• a bacteriphage

• a mutated gene sequence that will be destroyed permanently

CRISPR is composed of

• the cas9 protein and guide RNA

• RNA and ribosomes

• bacteria and viruses

• cas9, cas 1+2 and ribosomal RNA

What does the cas9 protein do?

• cut viruses

• Cut RNA

• cut DNA

• ingest bacteria

What does the guide RNA do?

• destroy invaders like bacteriophages immediately

• cut invading pieces of RNA

• recognize invaders

• recognize sequences of DNA to be edited.

What is the first step in using CRISPR?

• create an important RNA sequence

• find bacteriophages to invade bacteria

• identify the sequence causing the health problem

• create a virus