SERUM INDICES: MANAGING ASSAY INTERFERENCE

Which of the following analytical phase in clinical laboratory testing produce the highest frequency of errors?

• Analytical Phase

• Post-Analytical Phase

• Pre-Analytical Phase

The sources of endogenous interference include the following substances EXCEPT?

• Bilirubin

• Drugs

• Hemoglobin

• Lipids

Hemolysis is defined as breakdown of Leukocytes in blood which release hemoglobin & intracellular contents from cells. TRUE or FALSE ?

• TRUE

• FALSE

Which of the following is NOT mechanism of haemolysis interference?

• Chemical reaction of heme or its iron with reagent components

• Falsely increase in analytes concentration released from intracellular.

• Light scattering effect due to large size of lipoproteins or particulate matter

• Spectral interference of Hemoglobin at 340-440 nm & 540-580 nm.

''The yellowish serum or plasma colour resulting from increased sample bilirubin concentration. ''

The above statement show the definition of ?

• Hemolysis

• Lipaemic

• Icterus

The following factors contribute to in vitro haemolysis in blood sample EXCEPT?

• Traumatic collection

• Errors in sample handling

• Inherited hemolytic anemia

• Collection of blood

The following answers show the strengths of automated measurement of serum indices.

• Consistent than human visual

• Less Accurate compare to human visual

• Increase Laboratory Turn Around Time (LTAT)

• Enhanced accuracy of reportable test results.

What kind of interference present in this sample?

• Icteric

• Lipaemic/Turbidity

• Hemolysis

Choose the correct cause(s) of Lipaemic sample.

• Recent fatty meal

• Diabetes mellitus

• Medication

• High cholesterol intake

Among these interference substances, which is the common pre-analytical error affecting biochemistry results?

• Lipamia

• Hemolysis

• Icterus