MI 1.1.3 DNA Sequencing Check-Up

Which technique(s) that you've already seen (briefly) are a part of the DNA sequencing process? (more than one correct answer)

• restriction enzyme digest

• PCR

• electrophoresis

• blood typing

What is the correct sequence that corresponds with this DNA sequencing chromatogram?

• TCATGGCA

• AGCACCGT

• CACGTTGC

• CTGCAACA

How are regular nucleotides and dideoxynucleotides different? (more than one correct answer)

• Regular nucleotides are fluorescently labeled, but dideoxy nucleotides are not.

• Dideoxy nucleotides stop the elongation of the DNA strand during PCR.

• Dideoxy nucleotides are for making RNA, but regular nucleotides are for making DNA.

• Dideoxy nucleotides are fluorescently labeled, but regular nucleotides are not.

What happens in that skinny capillary tube in the automatic DNA sequencer machine? (more than one correct answer)

• The DNA fragments migrate towards the positive electrode at the bottom.

• The DNA fragments separate according to their size.

• The DNA fragments have a laser shined on them to excite the fluorescent dideoxy nucleotides.

• The machine detects and records what color the DNA fragment is.

In the end, how do you find out which pathogen matches your DNA sample?

• Search for a match using the NCBI BLAST website.

• Look at the sample under the microscope.

• Google it.

• Print out your sequence and compare it to sequences published in scientific journal articles.

Place the following processes for using DNA for bacterial identification in order:

• 1, 4, 3, 5, 2

• 1, 2, 5, 3, 4

• 1, 4, 5, 2, 3

• 1, 2, 3, 4, 5

Which body system is most affected by Meningitis?

• Circulatory System

• Endocrine System

• Nervous System

• Muscular System

What type of pathogen caused Sue's meningitis?

• bacteria

• virus

• protozoan

• fungus