Microscope Use

Magnifies the image (usually 10x) and you look into the scope here.

• Eyepiece/ Ocular
• arm
• low power objective
• fine adjustment

Moves body tube slightly to sharpen the focus of the image

• arm
• coarse adjustment
• eyepiece/ ocular
• fine adjustment

Longest objective (40x); Has the smallest field of view; Most detail; *Never use the coarse adjustment knob*

• low power objective
• high power objective
• scanning objective
• eyepiece/ ocular

Find total magnification for:
Eyepiece = 20X 
Objective lens = 20X  

• 20x
• 40x
• 200x
• 400x

How do you determine the total magnification of your image?

• Add the magnification of the eyepiece to the objective you are using.
• Add the magnifications of all three objectives on your microscope.
• Multiply the magnifications of all three objectives on your microscope.
• Multiply the magnification of the eyepiece and the objective you are using.

Never use this knob on high power.

• Fine Adjustment
• Large Focus
• Coarse Adjustment
• Small Focus

When holding the microscope, you should have one hand under the base and the other ______

• Holding the body tube
• Holding the stage
• Holding the arm
• Holding the objectives

Which focus do you use first to find your specimen on low power?

• Fine

• Course

• Neither

The _______________ is the large knob used to focus on the specimen. It moves the stage part of the microscope up and down. This is only used on low power.

• fine adjustment knob

• coarse adjustment knob