PCR

What does PCR stand for?

• Polymerase Chronic Reagent
• Principle Chorionic Reliability
• Polymerase Chain Reaction
• Probably Cannot React

From where does Taq polymerase come?

• Bacteria that live in hydrothermal vents/hot springs.

• Cambodian humans

• Created and synthesized by lab technicians

• The CDC

At what temperature does the Denature step of PCR occur?

• 94-96° C
• 50-65° C
• 72° C
• Any temperature

Tags

NGSS.HS-PS1-2

NGSS.HS-PS1-5

At what temperature does the Anneal step of PCR occur?

• 94-96° C
• 50-65° C
• 72° C
• Any temperature

At what temperature does the Extension step of PCR occur?

• 94-96°C
• 50-65°C
• 72°C
• Any temperature

What is the function of a primer?

• To identify the particular region of DNA to be copied by PCR.
• To copy DNA.
• To create DNA nucleotides.
• To maintain the temperature of the PCR reaction.

Why does PCR require a specific type of polymerase?

• Taq polymerase can withstand the range of temperatures in PCR without denaturing.
• Taq polymerase is the most inexpensive type of polymerase.
• Taq polymerase is the most common type of polymerase.
• A specific type of polymerase is unnecessary.

PCR happens in 3 steps (denaturing, annealing, extending) or 1 "cycle". How many times are cycles typically repeated?

• 1
• 10
• 30
• Over 1 billion

You decide to use PCR to determine if you have Cystic Fibrosis, a genetic disorder. You then decide to also check if you are at risk for developing Alzheimers, another genetic disorder.
What PCR "ingredient" would be different in these two tests?

• Template DNA
• Nucleotides
• Taq DNA polymerase
• Primers

What happens in the Denature step of PCR?

• The DNA nucleotides are broken apart.
• The base-pairing rules for DNA are reversed.
• The double-stranded DNA is separated into two single strands of DNA.
• The DNA is returned to its natural setting.