BIOCATALYSIS

Which of the following bonds can be found in enzyme?

• Disulphide bridges

• Hydrogen bonds

• Peptide bonds

• Ionic bonds

Which of these are properties of enzymes?

• Reusable

• Needed in small quantity

• Highly specific

• Fibrous protein

Explain model shown above

• The active site is exactly complementary to substrate

• The active site is highly specific

• The active site is flexible

• The active site is rigid

State the function of X

• Binding site of substrate

• Binding site of cofactor

• Binding site of reactant

• Binding site of inhibitor

How does enzyme lower the activation energy?

• Bring substrate closer to each other

• Increase the kinetic energy of substrate

• Weakening the bonds inside the substrate

• Bring substrate together into correct orientation

Enzyme that join the ends of two strands of nucleic acid is

• Ligases

• Hydrolases

• Transferases

• Isomerases

Decarboxylase is an example for

• Oxidoreductases

• Transferases

• Lyases

• Isomerases

Explain the graph

• Enzymes are denatured

• Enzyme's active site are fully occupied with substrate

• Active site of enzyme is no longer complementary with substrate

• The rate of reaction increases with increasing substrate concentration

Describe what happen to Pepsin when it is placed in basic environment

• Hydrogen bonds, ionic bonds, disulphide bridges,hydrophobic interactions and Van Der Waals forces break

• Conformation of the enzyme's active site changed

• Hydrogen bonds and ionic bonds break

• Enzyme-substrate complex unable to be formed

Describe the effect of temperature as it increase

• kinetic energy of substrate and enzyme increased

• rate of reaction is maximum

• collision between substrate and enzyme's active site increased