AP Biology DNA Practice Test

AP Biology DNA Practice Test

11th - 12th Grade

31 Qs

quiz-placeholder

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AP Biology DNA Practice Test

AP Biology DNA Practice Test

Assessment

Quiz

Biology, Science

11th - 12th Grade

Practice Problem

Medium

NGSS
HS-LS1-4, HS-LS1-1, MS-PS1-1

+1

Standards-aligned

Created by

Tess Bernhard

Used 374+ times

FREE Resource

About this resource

This AP Biology DNA Practice Test comprehensively covers the molecular basis of heredity at the 11th-12th grade level, focusing on DNA structure, replication, transcription, translation, and cell division. Students must demonstrate mastery of complex biochemical processes including the semi-conservative model of DNA replication, the directional synthesis of leading and lagging strands, and the role of specific enzymes like DNA polymerase, primase, and ligase. The quiz requires deep understanding of Chargaff's rules, base pairing principles, and the structural components of nucleotides. Advanced topics include RNA processing in eukaryotes (5' capping, 3' poly-A tailing, and splicing), genetic code degeneracy, mutation effects, gene regulation through promoter sequences, and the central dogma of molecular biology. Students must also understand mitotic cell cycle phases, chromosome behavior during division, and cell cycle regulation through cyclins and CDKs. Created by Tess Bernhard, a Biology teacher in US who teaches grade 11-12. This assessment serves multiple instructional purposes, functioning effectively as a comprehensive review tool before AP Biology exams, formative assessment to gauge student understanding of molecular genetics, or homework assignment to reinforce classroom instruction on DNA and cell division. The quiz can be strategically deployed as a warm-up activity to activate prior knowledge before introducing related topics, or as targeted practice for students preparing for standardized assessments. This material directly supports AP Biology Learning Objectives 3.1-3.6 and 3.28, covering DNA replication mechanisms, gene expression regulation, and cell cycle control. The assessment aligns with NGSS standards HS-LS1-4 (modeling DNA to RNA to protein information flow) and HS-LS3-1 (asking questions to clarify gene expression relationships), providing teachers with valuable data on student mastery of these essential molecular biology concepts.

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31 questions

Show all answers

1.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

How do the leading, and the lagging strands differ?

The leading strand is synthesized in the same direction as the movement of the replication fork, and the lagging strand is synthesized in the opposite direction.
The leading strand is synthesized at twice the rate of the lagging strand.
The leading strand is synthesized in short fragments that are ultimately stitched together, whereas the lagging strand is synthesized continuously. 
The leading strand is synthesized by adding nucleotides to the 3' end of the growing strand, and the lagging strand is synthesized by adding nucleotides to the 5? end. 

2.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Suppose a double-stranded DNA molecule was shown to have 15% adenine bases. What would be the expected percentage of guanine bases in that molecule?

15%
35%
85%
Not enough information

3.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Media Image

Consider the replication bubble diagrammed at the right. Which letters represent leading strands? 

W and X
Y and Z
W and Z
X and Y

4.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Which enyzme would be found only on the lagging strand?

DNA polymerase
Primase
Ligase
Topoisomerase

5.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Which enzyme is not working correctly if new DNA bases are not added to the template strand?

DNA Polymerase
Topoisomerase
Ligase
Primase

6.

MULTIPLE CHOICE QUESTION

2 mins • 1 pt

What is meant that DNA replication follows a “semi-conservative” model?

After DNA replication, each strand contains one side that is a parental strand and the other side is a daughter strand.
After DNA replication, one completed strand contains only parental DNA while the other contains only daughter DNA.
None of these identify the “semi-conservative” model.
After DNA replication, one completed strand contains a mixture of both parental DNA and daughter DNA dispersed throughout the strand.

7.

MULTIPLE CHOICE QUESTION

1 min • 12 pts

Which RNA bases would pair with TACGAA in transcription

AUGCUU
ATGCTT
GCATCC
GCAUCC

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