Microbiology Sterilization and Incubation Quiz 2

Sterilization is best defined

• The removal of 99.99% of the pathogens

• the complete elimination of all microorganisms

• procedures to prevent contamination from pathogens

• the use of soap and either ethyl alcohol or isopropyl alcohol to kill most bacteria

• reduction in the number of bacteria, viruses, or fungi to a desired concentration

The basic principle of steam sterilization, as accomplished in a...

• oven

• incubator

• autoclave

• bunsen burner

• electric kettle (jug)

The temperature at which water boils at or near sea level is ________

• 10 °C

• 100 °F

• 100 K

• 100 °C

• 98.7 °C

True sterilization is determined as a function of time, temperature, and pressure. These variables may be slightly different depending item being sterilized and the type of bacteria you are trying to kill. However, most microbial cells will die at a temperature of _____ ºC.

• 0

• 10

• 50

• 100

At high altitudes (say the top of a mountain) water

• will never boil

• will boil but at a lower temperature

• will boil but at a higher temperature

• will boil at the same temperature as at sea level ... but it takes more time

• will boil at the same temperature as at sea level ... but it takes less time

Which process is the least effective in killing microbes

• sanitizing

• UV radiation

• gamma Radiation

• hot water and soap

• boiling for 10 minutes

The most difficult form of microorganisms to inactivate or "kill"

• fungi

• viruses

• parasites

• bacterial spores

• streptococcus bacteria

The diagram below is most like a ...

• incubator

• autoclave

• microwave

• washing machine

• automatic dryer

Which classification of bacteria would like be the most difficult to kill using dry heat or by boiling

• mesophiles

• thermophiles

• psychrophiles

• hyperthermophiles

The equipment in the diagram is set and 37°C and does not use water it is most likely a...

• incubator

• autoclave

• microwave

• toaster oven

• photospectrometer