Biotechnology Test

Bacterial DNA that is often circular or in the shape of a ring is call a:

• Clone

• Plasmid

• Fragment

• Recombinant DNA

When fragments of DNA are connected from different sources, this creates:

• A plasmid

• A clone

• Recombinant DNA

• Fragmented DNA

Organisms that have had DNA from other organisms inserted into their DNA to produce certain traits are called:

• Plasmids

• Clones

• Transgenic organisms

• Recombinant organisms

Which of the following is a medical application of genetic engineering?

• Inserting human DNA into bacterial DNA for insulin production

• Manipulating of DNA to produce glowing fish

• Engineering crops for greater nutritional content

• Modifying genes to achieve a desirable phenotype

Using genetic engineering to treat or cure diseases is called:

• Bacterial transformation

• Selective breeding

• Gene therapy

• Alternative medicine

Crops may be genetically modified for pesticide resistance. Which of the following practices is then unnecessary?

• Spraying with pesticides

• Irrigating crops

• Rotating crops

• Harvesting crops

Which of the following describes bacterial transformation?

• The use of viruses to transform or genetically engineer cells.

• The measure of how well cells are transformed in a new phenotype

• The insertion of a plasmid with a foreign gene into a bacterial cell which results in new acquired traits

• Genetic engineering or transformation of mammalian cells

A scientist wants to combine a bacterial plasmid with a human insulin gene.

• She should use the same restriction enzyme to cut them to make blunt ends

• She should use the same restriction enzyme to cut them to make sticky ends

• She should use different restriction enzyme to cut them to make blunt ends

• She should use different restriction enzyme to cut them to make sticky ends

What are the steps of bacterial transformation in order?

• 1. Cut insulin gene and plasmid with same restriction enzyme 2. wait for insulin gene to start making insulin 3. put the plasmid into the bacteria cell
• 1. Cut insulin gene and plasmid with same restriction enzyme 2. isolate insulin gene 3. combine insulin gene and plasmid at sticky ends to make a recombinant plasmid 4. insert the recombinant plasmid into a bacteria cell
• 1. isolate insulin gene 2. cut insulin gene and plasmid with same restriction enzyme 3. combine insulin gene and plasmid at sticky ends to make a recombinant plasmid 4. insert the recombinant plasmid into a bacteria cell
• 1. isolate plasmid 2. cut insulin gene and plasmid with same restriction enzyme 3. wait for insulin gene to start making insulin 4. combine insulin gene and plasmid at sticky ends to make a recombinant plasmid

Based on these results, whose blood was found in the blood stain at the crime scene?

• Bob

• Sue

• John

• Lisa