FS 5 - DNA Fingerprinting

Bob was found dead in his home.  Crime scene analysis showed signs of a struggle.  Blood was collected from the crime scene, the victim, and three suspects.  Which suspect likely committed the crime? 

• Sue
• Lisa
• John
• None of these

DNA fingerprinting works because

• Most genes are dominant
• All organisms contain RNA
• The most important genes are different among most people
• No two people, except identical twins, have exactly the same DNA 

What is the purpose of gel electrophoresis?

• helps cut DNA
• count the genes in DNA
• separates DNA based on size
• allows for an exact replicated organism

In gel electrophoresis, the largest DNA fragment will appear

• closest to the starting wells
• farthest from the starting wells
• three quarters away from the starting wells
• it depends on how many fragments there are

Which is the primary purpose of using restriction enzymes in gel electrophoresis?

• It allows the strands of DNA to be cut into various lengths for testing
• It restricts the number of base pairs that can be tested in a sample
• It makes the testing simpler by moving the strands into the gel faster
• It charges the DNA strands

A molecule labeled with radioactive isotope, dye or enzyme that is used to locate a particular sequence or gene.

• DNA Probe

• DNA Fingerprint

• Electrophoresis

A method used to rapidly make multiple copies of a specific segment of DNA.

• PCR (Polymerse Chain Reaction)

• Electrophoresis

• DNA Probe

A molecule that cuts a DNA molecule at a specific, base sequence.

• Allele

• Restriction Enzyme

• DNA Probe

Tandem repeats of short DNA sequences with varying numbers of repeats found among individuals.

• STR (Short Tandem Repeat)

• PCR (Polymerase Chain Reaction)

• VNTR (Variable number of Tandem Repeats)

The purpose of PCR is to:

• Cut DNA
• Move DNA into a cell
• Analyze the length of DNA
• Copy short segments of DNA