Cloning Vectors

Restriction Enzymes are . . . 

• proteins
• DNA
• fats
• carbohydrates

Restriction Enzymes . . . 

• cut DNA at precise locations
• help bacteria to pick up DNA
• move DNA through a gel
• copy DNA

Some restriction enzymes produce "sticky ends". Sticky ends are . . .

• single-stranded DNA "overhangs" that can bind to complementary DNA

• double stranded DNA aligned with each other

• single-stranded DNA aligned with host DNA

• ends of a double stranded DNA strand

How do we name restriction Enzymes ?

• First letter in Cap for Genus, next two letter in small for species, next one letter for strain and roman numeral for order of discovery

• First two letter in Cap for Genus, next one letter in small for species, next one letter for strain and English numeral for order of discovery

• First two letter in Cap for Genus, next one letter in capital for species, next one letter for strain and roman numeral for order of discovery

• First two letter in Cap for Genus, next one letter in small for species, next one letter for strain and roman numeral for order of discovery

Which RE's from above make blunt cuts? (More than one answer)

• Alul

• HaeIII

• BamHI

• HindIII

• EcoRI

Which RE's from above make sticky cuts? (More than one answer)

• Alul

• HaeIII

• BamHI

• HindIII

• EcoRI

Restriction Enzymes were primarily used as....

• defense by protozoa against antibiotics

• tools by viruses to cleave bacterial genome

• defense by bacteria against bacteriophages viruses

• tools to cleave DNA during replication

What is a palindrome?

• A sticky end resulting from a restriction enzyme

• A blunt end resulting from a restriction enzyme

• A sequence that reads the same forwards and backwards

• A sequence that is complementary to the other

Identify the given plasmid.

• pUC19

• pBR322

• pUC119

• pUC18

What are selectable marker ?

• a radium labelled marker

• a type of sequence

• resistant gene

• a type of protein