Production of Insulin by Genetic Engineering

Which of the following cannot serve as an external source of insulin except by genetic engineering?

• Escherichia coli
• Sheep
• Goat
• Cow

What is a transgenic organism?

• an organism that has human DNA inside it
• an organism that has human insulin gene inside it
• an organism that has the gene or DNA of another organism inside it
• an organism that produces human insulin

Which type of diabetes cannot be treated using genetically engineered insulin?

• insulin dependent diabetes
• non-insulin dependent diabetes
• aggressive diabetes
• infectious diabetes

Human insulin produced by genetic engineering is better because (a) it acts faster (b) it lasts longer in the body (c) it does not cause allergic reactions

• a,b, and c
• a and c
• b and c
• a only

The structure from which insulin mRNA can be obtained is ………………………………

• pancreas
• islets of Langerhans
• blood plasma
• liver

which of the following component is not necessary to be included in forming recombinant DNA for insulin production by bacteria?

• antibiotic resistance marker
• eukaryotic promoter
• prokaryotic promoter

for individuals who have developed tolerance to animal insulin

• little amount of the insulin causes large unwanted reduction in blood glucose level
• their muscles and liver cells do not respond well to the insulin introduced
• insulin produced by genetic engineering may not be of much help to them
• insulin is destroyed by their phagocytes once introduced

Why is insulin produced by genetic engineering more rapid in its action?

• it is similar to animal insulin

• It has a shape that is exactly similar to that of natural human insulin

• It has higher optimum temperature of activity

• it is transported more rapidly by the blood to target sites

In producing insulin by genetic engineering, what is the first step to take?

• isolate insulin from pancreas

• isolate insulin mRNA from pancreas

• isolate insulin DNA from pancreas

• isolate insulin from animal tissue

mRNA is converted to cDNA by ………………

• Ligase enzyme
• Reverse Transcriptase enzyme
• Restriction Endonuclease enzyme
• RNA polymerase enzyme