Histopathology Dry Run

After death, how long does it take for a body to achieve complete hardening?

• 12 hours

• 16 hours

• 8 hours

• 24 hours

What is the temperature of a paraffin wax routine?

• 51 Celcius

• 42 Celcius

• 56 Celcius

• 60 Celcius

How thick is a cryostat cut?

• 4mm

• 5mm

• 6mm

• 3mm

In EPOS, how many molecules of enzymes can be attached to a dextrose polymer?

• 60

• 70

• 65

• 75

This is the most common clearing agent and it is toxic and therefore a threat to personnel working in histopathology laboratories.

• Toluene

• Xylene

• UltraClear

• Coconut Oil

Which organ tissue does not undergo coagulation necrosis?

• Brain tissue

• Liver Tissue

• Muscle Tissue

• Stomach Lining tissue

What is the TAT for frozen sections?

• 5-15mins

• 10-15mins

• 15-25min

• 10-20mins

What is the purpose of a cold running water in a trypsin method antigen retrieval?

• Unmask the antigens and epitopes in formalin-fixed and paraffin embedded tissue sections

• Termination of enzyme digestion

• Break the protein cross-links

• Enhancing staining intensity of antibodies

What destroys endogenous peroxidase?

• 0.5% Pentanolic Hydrogen Peroxide

• 0.3% Hydrogen Peroxide

• 0.36% Beta-glucose

• 0.013% Sodium Azide

Tissues embedded in celloidin are usually sectioned with a _______ microtome.

• Rotary

• Sliding

• Base Sledge

• Cambridge rocking

• 2 of these microtomes are applicable