Proteomics - 2D gel electrophoresis

In electrophoresis, DNA will migrate towards

• cathode or positive electrode

• anode or negative electrode

• cathode or negative electrode

• anode or positive electrode

The electrophoresis technique that used isoelectric focusing is

• AGE

• 2D-PAGE

• PFGE

• SDS-PAGE

Which technique separates charged particles using is

• Hydrolysis

• Electrophoresis

• Protein synthesis

• Protein denaturing

If proteins are separated according to their electrophoretic mobility then the type of electrophoresis is

• SDS PAGE

• Free flow electrophoresis

• Affinity Electrophoresis

• Electro focusing

When is electrophoresis not used?

• Separation of proteins

• Separation of nucleic acids

• Separation of Lipids

• Separation of amino acids

What cannot be a reason for using electrophoresis?

• Comparing two sets of DNA

• Organizing DNA by shape of backbone

• Organizing DNA fragments from largest to smallest

• Organizing DNA in order we can see

In an SDS-PAGE

• proteins are denatured by the SDS

• smaller proteins migrate more rapidly through the gel

• proteins have the same charge-to-mass ratio

• all of the above

In isoelectric focusing, proteins are separated on the basis of their

• size

• relative content of positively charged residue only

• relative content of negatively charged residue only

• relative content of positively and negatively charged residue

Proteins can be visualized directly in gels by

• measuring their molecular weight

• using electron microscope only

• staining them with the dye

• none of these

In a gel filtration column

• smaller proteins enter the beads more readily

• large proteins elute first

• both (1) and (2)

• large proteins enter the beads more readily