Microscopy-Bright field, dark field, phase contrast

Calculate the total magnification of microscope with objective lens of 40 x and ocular lens of 10 x

• 200x

• 100x

• 40x

• 400x

Which is correct about dark field

• Background is bright and microbial cells are dark

• Background is dark and microbial cells are bright

• Background is dark

• Background is bright and microbial cells appear stained

Phase contrast microscope was invented by

The undiffracted light is

• decreased by lamba/4

• no change

• increased by lamba/4

• decreased by lamba/2

Tags

NGSS.HS-PS4-1

Immunoflourescence is an application of

• Bright Field

• dark Field

• Phase contrast

• Flourescence Micrscopy

Tags

NGSS.HS-PS4-3

NGSS.HS-PS4-5

The system which increases or decrease phase changes of undiffracted and diffracted light is called

• Condenser annulus

• Objective lens

• Phase plate

• None of the above

The chemical dyes used to stain biological molecules for florescence is called

• Florophore

• Florochrome

• Giemsa

• DAPI

Which component of fluorescence microscope allows only light falling in absorption range of florophore to enter sample

• Emission Filter

• Barrier filter

• Dichroic mirro

• Excitation Filter

Anton Van Leeuwenhoek called moving microbes as

What is the resolving power of Transmission electron microscope

• 10 nm

• 200 nm

• 100 nm

• 0.2 nm