PCR

What is the purpose of PCR?

• To produce millions of copies of DNA.
• To produce millions of copies of a specific region of DNA
• To add nucleotides to a DNA sequence
• To watch polymerase work.

What does PCR stand for?

• Polymerase Chronic Reagent
• Principle Chorionic Reliability
• Polymerase Chain Reaction
• Probably Cannot React

From where does Taq polymerase come?

• Bacteria that live in hydrothermal vents/hot springs.
• Cambodia
• The rain forest
• The CDC

At what temperature does the Denature step of PCR occur?

• 94-96° C
• 50-65° C
• 72° C
• Any temperature

At what temperature does the Anneal step of PCR occur?

• 94-96° C
• 50-65° C
• 72° C
• Any temperature

At what temperature does the Extension step of PCR occur?

• 94-96°C
• 50-65°C
• 72°C
• Any temperature

What is the function of a primer?

• To identify the particular region of DNA to be copied by PCR.
• To copy DNA.
• To create DNA nucleotides.
• To maintain the temperature of the PCR reaction.

Why does PCR require a specific type of polymerase?

• Taq polymerase can withstand the range of temperatures in PCR without denaturing.
• Taq polymerase is the most inexpensive type of polymerase.
• Taq polymerase is the most common type of polymerase.
• A specific type of polymerase is unnecessary.

What are the base-pairing rules in DNA?

• A-C, G-T
• A-U, G-C
• A-T, G-C
• A-C, G-U

PCR happens in 3 steps (denaturing, annealing, extending) or 1 "cycle". How many times are cycles typically repeated?

• 1

• 5-10

• 25-30

• Over 1 billion