Restriction Endonucleases and Genetic Engineering

What is the role of restriction endonucleases in recombinant DNA technology?

• Synthesizing mRNA from DNA

• Cutting DNA at specific recognition sites

• Joining DNA fragments together

• Initiating transcription in a gene

Which of the following is a characteristic of restriction enzymes?

• They cut DNA at random locations

• They cut only single-stranded RNA

• They recognize specific palindromic sequences in DNA

• They add nucleotides to the ends of DNA fragments

Which of the following is an example of a restriction enzyme?

• DNA polymerase

• Ligase

• EcoRI

• Reverse transcriptase

What type of ends are produced by restriction enzymes like EcoRI?

• Blunt ends only

• Sticky ends only

• Both blunt and sticky ends

• No ends, as it does not cut DNA

Which statement about restriction endonucleases is false?

• They act as molecular scissors in genetic engineering

• They occur naturally in bacteria

• They always cut DNA at random sites

• They are used in recombinant DNA technology to cut and insert genes

What is the function of reverse transcriptase?

• Synthesizing cDNA from mRNA

• Cutting DNA into smaller fragments

• Adding sticky ends to DNA fragments

• Initiating DNA replication

Reverse transcriptase is commonly used in genetic engineering to create which type of DNA?

• Double-stranded DNA from single-stranded DNA

• Complementary DNA (cDNA) from mRNA

• Messenger RNA from DNA

• Plasmid DNA from chromosomal DNA

Reverse transcriptase is naturally found in which type of organisms?

• Bacteria

• Viruses

• Plants

• Fungi

Why is cDNA preferred over genomic DNA in cloning eukaryotic genes?

• cDNA does not contain introns

• cDNA contains only exons and regulatory sequences

• cDNA can be easily transcribed into RNA

• cDNA is longer and more stable than genomic DNA

Which of the following enzymes is NOT involved in the process of making recombinant DNA?

• Reverse transcriptase

• DNA ligase

• RNA polymerase

• Restriction enzyme