Spectrophotometry Zoom Meeting

What two variables will you be measuring during the spectrophotometry lab?

• concentration and wavelength

• wavelength and absorbance

• absorbance and concentration

• wavelength and transmittance

A purple Kool-Aid, which is dyed with true purple dye, would reflect ________ colored and absorb _____ colored light. (separate two answers with comma)

(a)  

The absorbance values must fall between ___ and ___ on a calibration curve. (separate to answers with comma)

(a)  

If you dilute 10.0 mL of a 15.0 ppm Kool-Aid sample to produce a 20.0 mL sample, the final solution will have a concentration of (a)   ppm.

Briefly describe how a serial dilution is completed. (Include specific pieces of glassware that must be used)

During the spectrophotometry lab you will be measuring the absorbance at max. wavelength of (a)   (number) Kool-Aid samples

The waste generated during this lab will go

• in the liquid waste disposal

• down the sink

• back into the stock containers

The two standards analyzed during this lab are

• Red 1 and Blue 40

• Red 20 and Blue 1

• Red 40 and Blue 2

• Red 40 and Blue 1