Revision - Electrophoresis

Which technique separates charged particles using electric field?

• Hydrolysis

• Electrophoresis

• Protein synthesis

• Protein denaturing

Electrophoresis was developed by:

• Tswett

• Tsvedberg

• Tiselius

• Sanger

The speed of migration of ions in electric field depends upon:

• Shape and size of molecule

• Magnitude of charge and shape of molecule

• Magnitude of charge shape and mass of molecule

• Magnitude of charge and mass of molecule

Which of the following statements is true about migration of biomolecules?

• The rate of migration is directly proportional to the resistance of medium

• Rate of migration is directly proportional to current

• Low voltage is used for separation of high mass molecules

• Rate of migration is inversely proportional to current

What does the electrophoresis apparatus consist of?

• Gel, buffer chamber and fire pack

• Buffer chamber and electrophoresis unit

• Electrophoresis unit and gel separator

• Power pack and electrophoresis unit

If proteins are separated according to their electrophoretic mobility then the type of electrophoresis is:

• SDS PAGE

• Affinity Electrophoresis

• Electro focusing

• Free flow electrophoresis

Which of the following factors does not influence electrophoretic mobility?

• Molecular weight

• Shape of molecule

• Size of molecule

• Stereochemistry of molecule

When is electrophoresis not used?

• Separation of proteins

• Separation of amino acids

• Separation of Lipids

• Separation of nucleic acids

Which of the following statements about Western Blotting is correct?

• The detection of a particular protein by Western Blotting relies on the very specific interaction between the protein and its antibody.

• The detection of a particular protein by Western Blotting relies on labelling the protein with a specific dye.

• The detection of a particular protein by Western Blotting relies on labelling the antibody with a specific dye.

• The detection of a particular protein by Western Blotting relies on the denaturation of the protein.

If the molecules to be separated are larger than the size for conventional electrophoresis, which type of gels can be used?

• Wedge gels

• Buffer-gradient gels

• Pulse field gels

• Varying the amount of agarose will carry out the separation