RECOMBINANT DNA TECHNOLOGY

Which is true about plasmids?

• Plasmids can be cut at specific sequences called restriction sites.

• Plasmids contain origin of replication

• Plasmids can contain an antibiotic resistance gene.

• All of the statements are true about plasmids.

Which of the following terms describes the process of the uptake of recombinant plasmids into bacteria cells?

• amplification

• insertion

• transformation

• screening

Which enzyme helps join the foreign DNA into the new DNA?

• DNA Polymerase

• DNA ligase

• Restriction enzyme

• Reverse transcriptase

The cell that able to receive recombinant DNA for cloning purpose known as

• vector

• target DNA

• plasmid

• host cell

To produce desired protein _____________ should be taken from any source like human, animal and plant.

• recombinant DNA

• Target DNA

• plasmid

• DNA ligase

Which of the following enzymes can cut nucleic acids?

• DNA polymerase

• restriction endonuclease

• modifying enzymes

• reverse transcriptase

Which restriction enzyme listed below was isolated from E. coli bacteria?

• EcoRI

• HindIII

• HaeII

• SmaI

Which of the following is NOT a tool used in genetic engineering?

• plasmid

• restriction enzyme

• DNA helicase

• DNA ligase

What type of cut is this photo?

• Blunt

• sticky

During insertion step,

• The recombinant plasmid is inserted into a host cell

• The gene of interest is inserted into a plasmid

• The plasmid is inserted into the gene of interest