Recombinant DNA Technology

An enzyme that cut DNA molecules at a specific “recognition site” is called

• Restriction enzyme

• DNA polymerase

• RNA polymerase

• Taq polymerase

Which of the following sequences in double stranded DNA is most likely to produce sticky end fragments when treated with a restriction enzyme

• 
• 
• 
• 

In order to obtain the gene of interest from the DNA of organism,

• The DNA must be cut only at one site near the gene of interest

• The DNA must be cut at two sites to get a DNA fragment that contains the gene of interest.

• The DNA must be cut at any two sites to get any DNA fragments from the organism

• The DNA must not be cut at all, so that the gene of interest is retained

A cloning vector is

• An agent that transfers a pathogen

• An agent that transfers a foreign gene from one DNA molecule to another

• An agent that transfers a DNA segment to a RNA molecule

• An agent that transfers a RNA segment to a DNA molecule

Plasmid are important in biotechnology because they are

• a vehicle for the insertion of foreign DNA into bacteria

• surfaces for respiratory process in bacteria

• recognition site on recombinant DNA strand

• enzymes that cut DNA into restriction fragments

Which of the following statements describes a modifying enzyme?

• It cuts the DNA to obtain the gene of interest

• It breaks the DNA molecule to produce multiple fragments

• It stimulates the replication of a recombinant DNA

• It seals the inserted gene of interest to an open plasmid

Which of the following is TRUE regarding screening by using X-gal?

• Antibiotics will prevent the growth of bacteria without plasmid

• Bacteria cells with recombinant plasmids are resistant to antibiotics and are unable to hydrolyse X-gal

• Bacteria cells which contain non-recombinant plasmids are resistant to antibiotics and unable to hydrolyse X-gal

The process where a recombinant plasmid is inserted into a host cell is called ....

Identify the palindromic base sequence for EcoRI

Which genetic marker will be altered if a plasmid accepted a gene of interest during cloning process?

• LacZ gene

• Ampicillin resistant gene

• LacY gene

• LacA gene