Revision Recombinant DNA Technology

Restriction enzymes are

• DNA unwinding enzymes

• DNA joining enzymes

• DNA cleaving enzymes

• DNA synthesis enzymes

The enzyme used to join the DNA fragments

• Topoisomerase

• DNA ligase

• DNA polymerase

• Reverse transcriptase

Which one or more of these can be used as a vector?

i. Bacterium ii. Plasmid iii. E. coli iv. Bacteriophage

• i & ii

• ii & iii

• i & iii

• ii & iv

An organism that has had its genes manipulated by genetic engineering is considered as ___.

• transgenic

• crossbred

• transmutant

• culitvars

Which is NOT an advantage of modern genetic manipulation?

• It allows scientists to manipulate genetic features by breeding animals with desired traits.

• It allows scientists to research for possible cures to genetic diseases.

• It allows scientists to have direct influence over an organism's genes.

• It allows scientists to introduce novel genes into an organism.

Which of the following is not part of the normal process of cloning recombinant DNA in bacteria?

• restriction endonuclease cleaving gene of interest and plasmid.

• production of recombinant DNA using DNA ligase and a mixture of cut gene of interest and plasmid.

• transformation of bacteria by the recombinant DNA plasmids and selection using ampicillin.

• probing blots of bacteria clones with radioactive DNA complementary to the desired gene.

Which of the following tools of recombinant DNA technology is INCORRECTLY paired with one of its uses?

• restriction endonuclease - production of DNA fragments for gene cloning.

• DNA ligase - enzyme that cuts DNA, creating sticky ends.

• reverse transcriptase - production of cDNA from mRNA.

• Taq polymerase - copies DNA sequences in the polymerase chain reaction.

5'GAATTC3' is a restriction site widely used by restriction enzyme which produces sticky ends. The enzyme is

• BamHI

• HindIII

• EcoRI

• SmaI

Which of the following is correct sequence of process in recombinant DNA technology?

1. Isolation of target DNA & vector
2. Introducing recombinant DNA into host cell
3. Fragmentation of target DNA & vector by restriction endonuclease
4. Ligation of DNA fragment into vector
5. Culturing in host cell to get desired products.

• 1,2,3,4,5

• 1,3,4,2,5

• 1,3,4,2,5

• 1,4,3,2,5

Which of the following is used as selectable marker?

• Ampicillin resistance gene

• Plasmid resistance gene

• Salmonella resistance gene

• Penicillin resistance gene