ELISA

When a positive + result occurs in an ELISA test, then one could conclude that...

• a + result always means illness

• the antibody is present

• it is a false + and no illness

• an enzyme is present

What is a primary antibody?

• the second antibody used to detect foreign particle
• particle produced by the antigen
• the first antibody used to detect foreign particle
• second cell produced by the macrophage

Why is it necessary to wash the samples repeatedly?

• it is important to keep the lab wells clean
• it is important to wash away all bound and unbound antibodies from teh wells
• it is important to wash away the unbound antibodies
• washing the wells is just solid lab practice

The basic 4 steps of the ELISA are:
bind sample to well
add primary Ab and wash
add secondary Ab/enzyme and wash
add substrate

• TRUE
• FALSE

What does the S in ELISA stand for?

• substrate
• stationary
• sorbent
• starch

Why is there an enzyme attached to the secondary antibody?

• They link to the proteins used

• The catalyze reactions

• They provide a colormetirc indication of antigen exposure

• They are used to detect DNA

Antibodies not only protect us from infections, they can also be used in laboratories to determine whether or not a patient has or has had a disease.

• TRUE
• FALSE

In an ELISA assay, ________ and ________ are added to facilitate a color change.

• antibodies and antigens
• primary antibodies and secondary antibodies
• enzymes and substrates
• antigen and enzymes

Sue Smith was inflicted with 

• viral meningitis
• mononucleosis
• bacterial meningitis
• influenza H1N1

A sample of spinal fluid is taken from a college student suspected to have contracted spinal meningitis.Which diagnostic test will confirm the diagnosis fastest?

• Culturing the spinal fluid and testing for bacterial colony growth in a Petri dish.

• An ELISA Assay to determine the presence of menigitis antigens.

• DNA sequencing

• Protein Chromatography