DNA Technology

A restriction enzyme is used to cut both the gene of interest and the plasmid at the restriction sites.

• True
• False

In bacteria, restriction enzymes are used for defense. They cut up the DNA of a virus that infects bacteria.

• True
• False

Once the gene and plasmid have been cut with a restriction enzyme, another enzyme called _________ is used to join the pieces of DNA together.

• polymerase
• ligase
• dehydrogenase
• lactase

Which of the following terms describes the process of the uptake of recombinant plasmids into bacteria cells?

• rDNA technology
• transferrence
• transformation
• rDNA plasmid

The cell that able to receive recombinant DNA for cloning purpose known as

• vector

• target DNA

• plasmid

• host cell

Which of the following is NOT a tool used in genetic engineering?

• plasmid

• restriction enzyme

• DNA helicase

• DNA ligase

What type of cut is this photo?

• Blunt

• sticky

From where does Taq polymerase come?

• Bacteria that live in hydrothermal vents/hot springs.

• Cambodia

• The rain forest

At what temperature does the denaturation step of PCR occur?

• 95° C

• 50° C

• 72° C

Tags

NGSS.HS-PS1-5

Which of the following best describes the role of primers?

• Frame the beginning and end of the target sequence
• Copy the DNA 
• Cause DNA strands to separate
• Heat the sample