Genetic Engineering

DNA strands can be clipped crosswise at selected positions by using enzymes called

• palindromes

• DNA polymerases

• restriction endonucleases

• ligases

Which technique will hopefully be used to identify and devise treatments for diseases based on the genetic profile of the disease?

• genetic engineering

• PCR

• antisense technology

• microarray analysis

The polymerase chain reaction (PCR) has been used in all of the following fields, except

• gene mapping

• forensics

• medicine

• none of the choices; it is used for all these fields

Which PCR step causes the denaturation of double-stranded DNA?

• Repeat the cycle of heating and cooling

• Add primers

• Heat target DNA to 94 degrees Celsius

• Cool DNA to between 50 degrees and 65 degrees Celsius

The use of an organism's biochemical processes to create a product is

• Bioremediation

• gene probes

• biotechnology

• recombinant DNA

Transgenic animals

• will pass genes on to their offspring

• commonly include mice

• can be engineered to become factories for manufacturing proteins

• All of these choices are correct

Recombinant strains of this organism are released to colonize plant roots to produce an insecticide to destroy invading insects.

• Pseudomonas fluorescens

• Thermus aquaticus

• Escherichia coli

• Saccharmonyces cerevisiae

All of the following are correct about DNA fingerprinting, except

• it cannot be used on specimens older than five years

• it can be used to analyze evidence when only minute amounts of DNA are found

• it is routine to use it as trial evidence in the U.S.

• it displays the unique pattern of an individual's DNA fragments

Which of the following drugs is/are produced by genetic engineering and approved for human use?

• tissue plasminogen activator

• Factor VIII

• Human growth hormone

• All of these choices are correct

Amplification of DNA is accomplished by

• Southern blot

• polymerase chain reaction

• gene probes

• All of these choices are correct