Biosafety and Synthetic Bio

Why was the BAX gene used in the lab?

• Most readily available

• Induction of apoptosis is the activity needed for treatment

• Induction of non-apoptosis is the activity needed for treatment

• It is most most easily manipulated in cancer patients

Why is a promoter needed?

• To recruit RNA polymerase

• To recruit DNA polymerase

• To recruit Topoisomerase

• To recruit Helicase

Which sequence is cut by the restriction enzyme BP clonase?

• attB1

• attP2

• Both

• Neither

How are donor vectors usually identified?

• ppatient

• pcancer

• pdonr

• pflk

What is Next generation sequencing used for?

• To predict future outcomes of offspring

• To determine functionality of a gene

• To measure gene expression

• To map out the human genome

What are the att sites?

• Specific sequence that act like ligase

• Specific sequences that are recognized and recombined by clonase

• Specific sequences that removed from the new assembled sequence

• Specific sequences that elongate and copy the DNA

At what temp was the clonase kept?

• 20 C

• 15 C

• 5 C

• 4 C

What techniques was used to introduce the new DNA into the bacteria

• Gel electrophoresis

• Denaturation

• Electroporation

• Exocytosis

How long did it take to incubate the single colony in the LB broth?

• 20 mins

• 1 hr

• 16 hrs

• 24 hrs

Why was it necessary to add a buffer to the spun down cells?

• To dissolve the cell membrane for DNA extraction

• To balance the sample back after lysis

• For resuspension

• All of the above