Recombinant DNA Technology

What does genome mean?

• All the genes of an organism

• All the proteins an organism can produce

• All the genetic material of an organism

• The DNA and RNA found in the nucleus

Which of these are needed PCR? [select all correct answers]

• DNA polymerase

• DNA primers

• RNA primers

• Free DNA nucleotides

• Reverse transcriptase

Which enzyme does this?

• DNA polymerase

• Restriction enzyme

• Reverse transcriptase

• DNA ligase

What is shown in purple?

• Sticky ends

• Blunt ends

• Primers

• Restriction ends

What is produced by reverse transcriptase from mRNA?

• aDNA

• bDNA

• cDNA

• dDNA

What is a region of DNA that RNA polymerase attaches to to begin transcription.

• Transcriptional factor

• Terminator

• Start codon

• Promoter

What is the purpose of marker genes?

• To help transform cells

• To transfer a DNA fragment more easily

• To show which class have taken up vectors

• To identify if recombinant DNA has been taken up into cells

Which of there are common examples of marker genes? (select all correct answers)

• Fluorescent protein genes

• Dyed genes

• Antibiotic resistance genes

• Genes for enzymes

• Genes containing radioactive isotopes

Which of the following techniques might be used to transform bacterial cells?

• Bacteriophage

• High temperatures

• Ligation

• Cell wall digestion

What is a "machine that synthesises DNA fragments from scratch without the need for a preexisting template"

• DNA polymerazer

• Gene builder 5000

• Gene machine

• Genie