Biotechnology Review

This is the practice of producing a genetically identical cell or organism

• Cloning

• Gel Electrophoresis

• Genetic Engineering

• DNA Fingerprinting

This is the practice of cutting DNA and separating it by the size of the pieces

• Cloning

• Gel Electrophoresis

• Genetic Engineering

• DNA Fingerprinting

Which kitten is most likely the offspring of this cat?

• 1

• 2

• 3

• 4

When cloning an organism, DNA is removed and placed into an empty

• virus
• sperm cell
• bacteria cell
• egg cell

DNA fingerprinting works because

• Most genes are dominant
• All organisms contain RNA
• The most important genes are different among most people
• No two people, except identical twins, have exactly the same DNA 

What is the purpose of gel electrophoresis?

• helps cut DNA
• count the genes in DNA
• separates DNA based on size
• allows for an exact replicated organism

Why do the fragments of DNA in gel electrophoresis travel away from the negative electrode?

• DNA is negatively charged so attracted to the positive end of the unit
• DNA is positively charged to attracted to the negative end of the unit
• the agarose gel in negatively charged
• the agarose gel is positively charged

Which is the primary purpose of using restriction enzymes in gel electrophoresis?

• It allows the strands of DNA to be cut into various lengths for testing
• It restricts the number of base pairs that can be tested in a sample
• It makes the testing simpler by moving the strands into the gel faster
• It charges the DNA strands

A student performed a gel electrophoresis experiment. The results are represented in the diagram

below. Compared to the fragments at the top of the gel, the fragments at the lower end are

• larger, and move slower

• larger, and move faster

• smaller, and move faster

• smaller, and move slower

Once DNA has been extracted from the nucleus of a cell, it is exposed to ______ ______ that act like biochemical scissors to cut the DNA.

• restriction enzymes

• gel agarose

• clotting factors

• electric shock