BTC3003-HPLC

Which of the following is commonly used as a mobile phase in reverse-phase HPLC?

• a) Hexane

• b) Water-methanol mixture

• c) Acetone

• d) Sulfuric acid

In reverse-phase HPLC, the stationary phase is:

• a) Polar

• b) Non-polar

• c) Ionic

• d) Aqueous

Which parameter describes how well two compounds are separated in HPLC?

• a) Retention time

• b) Resolution

• c) Efficiency

• d) Peak height

Which detector is based on light absorption in HPLC?

• a) Refractive Index Detector

• b) Mass Spectrometer

• c) UV Detector

• d) Fluorescence Detector

The (a)   is the graphical output of the HPLC analysis.

Compounds eluting later in reverse-phase HPLC are more (a)   .

Increasing the (a)   can decrease retention time.

Poor separation between peaks may indicate low column (a)  

The (a)   column protects the main analytical column.

Column (a)   affects resolution and separation in HPLC.