Which of the following is commonly used as a mobile phase in reverse-phase HPLC?

• a) Hexane

• b) Water-methanol mixture

• c) Acetone

• d) Sulfuric acid

In reverse-phase HPLC, the stationary phase is:

• a) Polar

• b) Non-polar

• c) Ionic

• d) Aqueous

Which parameter describes how well two compounds are separated in HPLC?

• a) Retention time

• b) Resolution

• c) Efficiency

• d) Peak height

Which detector is based on light absorption in HPLC?

• a) Refractive Index Detector

• b) Mass Spectrometer

• c) UV Detector

• d) Fluorescence Detector

The ____________ is the graphical output of the HPLC analysis.

Compounds eluting later in reverse-phase HPLC are more ____________.

Increasing the ____________ can decrease retention time.

Poor separation between peaks may indicate low column ______________

The ____________ column protects the main analytical column.

Column ____________ affects resolution and separation in HPLC.