Aseptic Culture Transfer Techniques

When streaking a plate, the loop should be flamed

• At the beginning and end 
•  At the beginning and end and between the first 2 zones 
• At the beginning and end and between the second and third zone 
• At the beginning and end and between each zone 

Which finger should you use to remove the lid from a test tube when you are using an inoculating loop to make a transfer? 

• little finger of the dominant hand (hand holding the loop)
• little finger of the non-dominant hand (hand holding the sample)
• forefinger of the dominant hand (hand holding the loop)
• thumb and forefinger of the non-dominant hand (hand holding the sample)

When transferring liquid aseptically from one tube to another, you should

• remove the cap of the tube with the little finger of the hand holding the pipette
• remove the cap of the tube with the little finger of the hand holding the tube
• place the cap on the bench top with the inside up
• place the cap on the bench top with the inside down

What is the first thing you should do during aseptic culture transfer technique?

• put on gloves

• wash your hands

• sterilize your workspace

Which one of the following is the most appropriate disinfectant for cleaning the work area (Laminar Flow Cabinet)?

• saline

• water

• 70%ethanol

• 90%ethanol

Why is it important that the Petri plates are incubated upside down?

• To get dense colonies

• To get mat culture

• To get colonies with reduced growth

• To prevent moisture from condensing onto the agar surface

Flaming the neck of the test tube after uncapping the tube and before recapping is essential during culture transfer.

• True

• False

Name the instrument and its purpose.

What is Subculturing?

Why the culture transfer must be done aseptically?